SuperBLOK™ CoT Human DNA
SuperBLOK genomic DNA consists largely of rapidly annealing repetitive elements that can suppress cross-hybridization to repetitive DNA in microarray and in situ hybridization experiments. To enable specific hybridization of the probe to the chromosomal target site, the probe is denatured with a large excess of SuperBLOK DNA as a competitor. As a result the repetative regions are lost and the remaining DNA is then used as low copy probe.
- Specifications
- Applications
- Performance
- Quality
SuperBLOK™ CoT Human DNA |
MPNA-8888 | MPNA-8889 | MPNA-CUS |
CoT 1 | CoT 1 | Custom - COT 1 |
1000 ug (1mg) | 2000 ug (2mg) | Custom |
1 mg/ml | 2 mg/ml | Custom |
1 mL | 1 mL | Custom |
1mM EDTA, 10 mM Tris-HCl, pH 8.0 |
SuperBLOK™ DNA is stable at 2-6 °C for up to 4 months or at -20°C for longer storage. |
Applications
SuperBLOK™ CoT DNA consists largely of rapidly annealing repetitive elements that can suppress target probe cross-hybridization to repetitive DNA in microarray and in situ hybridization experiments. To enable specific hybridization to the chromosomal target site, the probe is denatured with a large excess of SuperBLOK CoT DNA as a compeititor. As a result the repetative regions are lost and the remaining DNA is then used as low copy probe.
Performance
SuperBLOK DNA has been optimized for a size range of 50 to 350 base pairs. Amount of DNA for effective supression of cross-hybridization depends on the type and amount of probe DNA. We recomment to start with a 50-100 fold excess of SuperBLOK DNA compared to probe DNA. Additionallly, microarray CGH performance can be improved by increasing hybridization time (16-72hrs).
0.5mg is ample for up to 10 Southern blot assays, or over 500 in-situ hybridization.
The optimum amount of SuperBLOK DNA for effective supression of cross-hybridization depends on the type and amount of probe DNA. We recomment to start with a 50-100 fold excess of SuperBLOK DNA compared to probe DNA.
Quality
All preparations are assayed for contaminating endonuclease and certifiably free of non-specific RNase, single and double-stranded DNase activities. MBI/GROWCELLS, Inc. takes extraordinary steps to assure that our SuperBLOK™ DNA does not exceed theoretical size maximums for repetitive DNA found in the genome of interest, while carefully
removing non-COT fragments which might block a real target site.
The human-source material used in the production of this procedure tested negative for hepatitis B virus, hepatitis C virus (HCV), human immunodeficiency virus type-1 (HIV-1) and type-2 (HIV-2), humanT-cell lymphotropic virus (HTLV-1 and HTLV-2) and Treponema pallidum. The product should be handled using standard Biosafety level 2 procedures. Handle as if potentially infectious. All tissues used are traceable to the birth source.
SuperBLOK™ is a trademark of MBI/GROWCELLS, Inc. Certain technologies and/or their methods of preparation, analysis or use may be covered by patents or other intellectual property rights held by others in certain countries. MBI does not encourage or support the unauthorized or unlicensed use of any technology. Use of SuperBLOK™ is recommended only for technology for which the end-user has a license under proprietary rights of third parties or for technology for which a license is not required. Cot-1 DNA is a registered trademark of Life Technologies, Inc.
This product is for RESEARCH USE ONLY. IT IS NOT INTENDED FOR DIAGNOSTIC APPLICATIONS.
Limited Product Warranty – Patent Disclaimer:
This warranty limits our liability to replacement of this product. No other warranties of any kind express or implied, including without limitation, implied warranties or fitness for a particular purpose, are provided by MBI/GROWCELLS, Inc. MBI shall have no liability for any direct, indirect, consequential, or incidental damages arising out of use, or the inability to use this product.
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