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Comparative Genomic Hybridization
Comparative Genomic Hybridization (CGH) is the prefered method for genome-wide analysis of DNA
[1]
that identifies small deletion and/or duplications in chromosomal regions. By having a labeled DNA of
interest hybridize with a labelled normal DNA, CGH generates a map of DNA with copy number changes
[2].
CGH has advanced in becoming essential in analzying chromosomal imbalances. However the sensitivity of
CGH can be thwarted by contaminants
[3] yielding false positives that can considerably slow down the process. Therefore MBI products are assayed for contaminating endonuclease
and optimized for all critical assays requiring the highest degree of specificity and purity.
DNA for Hybridization
SuperBLOCK DNA is an ideal suppressor of cross-hybridization or basis for construction of whole genomic libraries. Our DNA is extracted from U.S. sourced, karyotype verified, male birth, whole human placenta. The DNA is then prepared by organic solvent methods including phenol extraction to inactivate biological agents. SuperBLOK DNA purity, size, and concentration are verified by spectrophotometry and agarose gel electrophoresis.| MPNA-CUS | 1 mL | SuperBLOK™ CoT Human DNA |
| MPNA-8889 | 1 mL |
| MPNA-8888 | 1 mL |
| MPNA-7001 | 1 mL | SuperBLOK™ Whole Genomic Human DNA |
Hybridization
» Foot Notes
[1] Pinkel, D, et al. High resolution analysis of DNA copy number variation using comparative genomic hybridization to microarrays. Nature genetics, 20.2, 207-211 (1998)
[2] Kallioniemi, A. et al. Comparative genomic hybridization for cytogenetic analysis of solid tumors. Science 258, 818-821 (1992).
[3] Weiss, M.M, et al. Comparative genomic hybridisation. J Clin Pathol: Mol Pathol 52: 243-251 (1999)
