Culture Media Formulations

Standard formulations for liquid and powder culture media. All amounts are expressed per liter.

Item:Agar:Tryptone:Yeast Extract:Sodium Chloride (NaCl):K2HPO4:KH2PO4:
LB Miller's Agar Powder (40 g/L) 15g 10g 5g 10g    
LB Lennox Agar Powder (35 g/L) 15g 10g 5g 5g    
LB Miller's Broth Powder (25 g/L)   10g 5g 10g    
LB Lennox Broth Powder (20 g/L)   10g 5g 5g    
2X YT Broth Powder (31 g/L)   16g 10g 5g    
SuperBroth Powder (60 g/L)   35g 20g 5g    
Terrific Broth Powder (47.6 g/L)   12g 24g   9.4g 2.2g



Buffer Formulations

Standard formulations for buffers. All amounts are expressed per liter.


Item:Recipe/L:
0.1mM EDTA RNA Storage Solution0.03722g EDTA
0.5M Ammonium Chloride Solution (Sterile)26.76g Ammonium Chloride
0.5M EDTA, pH 8.0186.1g EDTA
0.5M EGTA Solution190.2g EGTA
10X PBS (Phosphate Buffered Saline) pH 7.4Sodium Chloride 80g, Potassium Chloride 2g, Sodium Phosphate Dibasic 14.4g, Potassium Phosphate Monobasic 2.4g
10X TAE (Tris-Acetate EDTA) pH 8.348.46g TRIS Base, 11.4ml Acetic Acid, 3.72g EDTA
10X TBE (Tris-Borate EDTA) pH 8.3Tris Base 108g, Boric Acid 55g, EDTA 9.3g
1M Calcium Chloride (CaCl2) Solution (Sterile)110.98 g CaCl2
1M LiCl (Lithium Chloride) Solution (Sterile)42.39g LiCl
1M Magnesium Acetate Solution142.39g Magnesium Acetate
1M MgCl2 (Magnesium Chloride) Solution (Sterile)95.2g Magnesium Chloride
1M MnCl2 (Manganese Chloride) Solution125.84g Manganese Chloride
1M Tris, pH 7.5121.14g Tris Base
1X PBS (Phosphate Buffered Saline) pH 7.4Sodium Chloride 8g, Potassium Chloride 0.2g, Sodium Phosphate Dibasic 1.44g, Potassium Phosphate Monobasic 0.24g
200mM Sodium Borate47g Boric Acid, 8g Sodium Hydroxide
20X PBS (Phosphate Buffered Saline) pH 7.4Sodium Chloride 160g, Potassium Chloride 4g, Sodium Phosphate Dibasic 28.8g, Potassium Phosphate Monobasic 4.8g,
20X SSC Buffer (Standard Sodium Citrate)175.3g NaCl, 88.2 Sodium Citrate
20X SSPE (3 M NaCl, 0.2 M NaH2PO4, 0.02 M EDTA)175.3g NaCl, 27.6g NaH2PO4-H2O, 7.4g EDTA
2M KCl (Potassium Chloride) Solution (Sterile)149g Potassium Chloride
3M Sodium Acetate Solution pH 5.5408.3g Sodium Acetate
50X TAE (Tris-Acetate EDTA) pH 8.3242.48g TRIS Base, 57ml Acetic Acid, 18.61g EDTA
5M Ammonium Acetate Solution (Sterile)385.4g Ammonium Acetate
5M NaCl (Sodium Chloride) (Sterile)292.2g NaCl
5M Potassium Acetate Solution490.75g Potassium Acetate
7.5M Ammonium Acetate Solution (Sterile)578.1g Ammonium Acetate
7.5M LiCl (Lithium Chloride) Solution (Sterile)317.93g LiCl
D-(+) Glucose, 20% Sterile SolutionUltrapure Glucose, 200 grams
D-(+) Glucose, 40% Sterile SolutionUltrapure Glucose, 400 grams
D-(+) Glycerol, 20% Sterile SolutionCertified Plant Derived Glycerol, 200 mL
Genomic Grade™ Isopropyl Alcohol>99.5% Purity Isopropyl Alcohol
LB Lennox Agar Powder (35 g/L)Agar 15g, Tryptone 10g, Yeast Extract 5g, NaCl 5g
PBS (Phosphate Buffered Saline), 0.05% Tween 20, 1X, pH 7.4Sodium Chloride 8g, Potassium Chloride 0.2g, Sodium Phosphate Dibasic 1.44g, Potassium Phosphate Monobasic 0.24g, 0.5ml Tween-20
Prolytic™ Proteinase - K Solution (20 mg/mL )20mg/ml
Sterile Saline 0.9% (NaCl) Solution9g NaCl
SuperBLOK™ CoT Human DNACustom Concentration
SuperBLOK™ CoT Human DNA2mg/mL
SuperBLOK™ CoT Human DNA1mg/mL
SuperBLOK™ Whole Genomic Human DNA1 mg/mL
SuperBroth Powder (60 g/L)Tryptone 35g, Yeast Extract 20g, NaCl 5g
SuperBroth Powder (60 g/L)Tryptone 35g, Yeast Extract 20 g, NaCl 5g
TE (Tris EDTA), 1X, pH 8.0 (Nuclease Free)10ml 1M Tris pH 7.5, 2ml 0.5M EDTA pH 8